tag:blogger.com,1999:blog-2878020709809350448.comments2017-11-30T09:35:30.794-05:00...And You Will Know Me By The Trail of PapersMikehttp://www.blogger.com/profile/13460212509225238067noreply@blogger.comBlogger61125tag:blogger.com,1999:blog-2878020709809350448.post-950388964839405472017-10-27T10:54:35.128-04:002017-10-27T10:54:35.128-04:00nice postnice post<a href="http://www.huffingtonpost.com/nancy-laws/the-shocking-truth-about-_5_b_7041934.html">custom writing service reviews</a>https://www.blogger.com/profile/05222531843183732336noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-6155461180619344212017-07-12T14:10:26.200-04:002017-07-12T14:10:26.200-04:00Hi there! I would really like to use this program,...Hi there! I would really like to use this program, but I am new to Python and I tried everything I could think of but it didn't work. I enlisted a Python veteran, but he also could not get the file to work. He recommended asking you for the file as an .exe. Can you send that to me to try? Thank you!! <br /><br />Molly McDougle<br />Research Assistant<br />John B. Pierce Laboratory<br />Anonymoushttps://www.blogger.com/profile/00824393772519188904noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-16725819560048970122017-05-23T01:47:32.601-04:002017-05-23T01:47:32.601-04:00Sorry, my lab only had a FLIR camera, I don't ...Sorry, my lab only had a FLIR camera, I don't know about Xenics.Mikehttps://www.blogger.com/profile/13460212509225238067noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-42266545806450053302017-05-22T09:19:13.131-04:002017-05-22T09:19:13.131-04:00Hi, one question, how could I get the temperature ...Hi, one question, how could I get the temperature from pictures fron a IR xenics camera? I can get only the video and pictures from the IR camera but not the range of temperatures.<br />I look forward for your answer<br />L.Unknownhttps://www.blogger.com/profile/13220694307354983402noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-52438078542096984172016-06-22T17:06:13.836-04:002016-06-22T17:06:13.836-04:00Hi Mike, This is PERFECT. Just what my client wil...Hi Mike, This is PERFECT. Just what my client will need. I'll forward.<br />Also, you could do me a huge favor. Can you possibly send me some raw data output files from BioDAQ. <br />If such files are under 10MB then send to wilant@gmail.com<br />If they are larger, then a Dropbox link would work, or www.wetransfer.com.<br /><br />THANKS MUCH IN ADVANCE!<br /><br /> Wilant van Giessen<brAnonymoushttps://www.blogger.com/profile/02122663652161944739noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-54410263750830696552016-06-17T02:01:33.424-04:002016-06-17T02:01:33.424-04:00I'm not sure I understand the question. If the...I'm not sure I understand the question. If there's no skull, there's no Bregma to worry about! If you're sectioning brains, and you're not sure where to start, you can start a few hundred microns away from where you think you should, and just make more sections.Mikehttps://www.blogger.com/profile/13460212509225238067noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-82114858284810851172016-06-14T17:04:42.272-04:002016-06-14T17:04:42.272-04:00What do you do if you have a frozen brain that is ...What do you do if you have a frozen brain that is not in the skull?Getting Hairy in Pittsburghhttps://www.blogger.com/profile/10252060220754532613noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-2790529971186225502016-04-20T06:53:36.039-04:002016-04-20T06:53:36.039-04:00nice post! thank yoU!nice post! thank yoU!Gargamellehttps://www.blogger.com/profile/13479224220236934749noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-69067336693577978302016-03-30T17:25:30.638-04:002016-03-30T17:25:30.638-04:00FLIR has some higher end video cameras, with highe...FLIR has some higher end video cameras, with higher resolution and frame rate. Of course, they run > $10,000.Anonymoushttps://www.blogger.com/profile/04983982147294139411noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-10541668401737410082016-03-30T13:13:53.202-04:002016-03-30T13:13:53.202-04:00S.V.S: maybe this would work for you:
http://peo...S.V.S: maybe this would work for you: <br /><br />http://people.csail.mit.edu/mrub/vidmag/joeyohttps://www.blogger.com/profile/11781088449143978381noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-34258133488254989172016-03-30T11:16:56.344-04:002016-03-30T11:16:56.344-04:00I'm thinking about both primates and rats ATM....I'm thinking about both primates and rats ATM. Flir C2 runs at 9Hz, so no dice there, even if the spatial res and sensitivity are good enough (which I don't know).s.v.shepherdhttps://www.blogger.com/profile/05305060453244526663noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-67437433560457658992016-03-29T19:06:39.538-04:002016-03-29T19:06:39.538-04:00We're mostly interested in thermoregulation, s...We're mostly interested in thermoregulation, so we just take one image every five minutes. I looked around, and FLIR sells a ~$400 model (C2) that allows you to stream video to a PC, but I have no idea what the framerate is. With mice that breath at 3-6Hz, I'd bet 30Hz would be ok. For primates, it's probably a lot easier.Anonymoushttps://www.blogger.com/profile/04983982147294139411noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-64010344171502936982016-03-29T14:00:39.459-04:002016-03-29T14:00:39.459-04:00Hi Mike. What's the frame rate like? Can you d...Hi Mike. What's the frame rate like? Can you detect respiratory fluctuations in temperature of the nose tip?<br />s.v.shepherdhttps://www.blogger.com/profile/05305060453244526663noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-26810684522397497362016-03-09T18:25:57.801-05:002016-03-09T18:25:57.801-05:00I have not used this system for a while, and I no ...I have not used this system for a while, and I no longer use Klustaview or NDmanager. But I can tell you what I do now. I switched to python a while ago, and I store my code for converting .ncs files on github at: <br />https://github.com/map222/MPNeuro/tree/master/nlxio<br /><br />To convert a bunch of .ncs files to a .dat binary, I change my current working directory to the directory with the Mikehttps://www.blogger.com/profile/13460212509225238067noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-51685067422908287252016-03-09T18:08:59.467-05:002016-03-09T18:08:59.467-05:00Hi Mike,
I was reading your blog cause I want to ...Hi Mike,<br /><br />I was reading your blog cause I want to do the same thing that you were doing here. I have data recorded by NeuraLynx and I want to use Klustaview for clustering the data. I need to change the data from .ncs to .dat but I am not sure how I can do it. NeuraLynx has a tool which can convert .ncs files to Matlab variables and then from this point I don't know how I can make Anonymoushttps://www.blogger.com/profile/07290707538230100973noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-8755946107204982492016-03-01T07:07:46.626-05:002016-03-01T07:07:46.626-05:00Great article! Extremely useful! Great article! Extremely useful! Cleitonhttps://www.blogger.com/profile/18140199667559508656noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-21218655290320040722016-01-25T17:20:22.929-05:002016-01-25T17:20:22.929-05:00Glad I could help! And to see I'm not the only...Glad I could help! And to see I'm not the only one who got tripped up by the flags.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-25086297967808524472016-01-25T15:56:41.304-05:002016-01-25T15:56:41.304-05:00Thank you for this post! Just downloaded some of t...Thank you for this post! Just downloaded some of their data and could not figure out how to actually get into it in Python; those record flags for loadmat were what I needed.<br /><br />-Gabe K. Ocker<br />Anonymoushttps://www.blogger.com/profile/00393057736448431263noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-49665963329691722002015-10-16T15:37:46.808-04:002015-10-16T15:37:46.808-04:00A new awesome spin on an old idea (Spin: Use ML, O...A new awesome spin on an old idea (Spin: Use ML, Old idea: on video games). Would be great if we could be watching a ranked game or worlds and the ML algorithm predicts the winner in real-time!DreamHackerhttps://www.blogger.com/profile/14163803338085348041noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-15551550726858738262015-06-11T14:29:54.160-04:002015-06-11T14:29:54.160-04:00Yup, subsets of AgRP neurons project to different ...Yup, subsets of AgRP neurons project to different brain regions, and stimulation of the fibres only some of the regions yields face-stuffing behavior.<br /><br />For peptides, it's thought that AgRP overlaps with NPY by >90%, but not much is known about other peptides. A couple labs are doing single cell sequencing to address this. There is also a developmental switch where some POMC Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-34521056240967512112015-06-11T09:25:41.337-04:002015-06-11T09:25:41.337-04:00I assume for the first case, these neurons may pro...I assume for the first case, these neurons may project to different areas? Or perhaps release different peptides!Adam Calhounhttps://www.blogger.com/profile/08980736131348018666noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-32970838829557723202015-05-15T16:31:39.790-04:002015-05-15T16:31:39.790-04:00Thanks for the link! It seems I still don't ha...Thanks for the link! It seems I still don't have the exact definition of bregma. I agree that the illustrations were idealized. I wish there was a resource with real pictures of mouse bregma that showed how to deal with non-ideal situations. I tried taking pictures myself, but couldn't get clean, high magnification images.Mikehttps://www.blogger.com/profile/13460212509225238067noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-86265133267474109512015-05-13T22:55:09.569-04:002015-05-13T22:55:09.569-04:00Thanks for this helpful post! One important thing ...Thanks for this helpful post! One important thing to point out, though, is that Paxinos defines bregma as:<br />"No atlas or stereotaxic instrument will compensate for using bregma and lambdoid points<br />inappropriately. These reference skull marks are the midpoints of the curve of best fit along the coronal and the lambdoid suture, respectively. They are not necessarily the points of Anonymoushttps://www.blogger.com/profile/01346418120410376007noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-19990450806927072202015-05-07T01:30:49.525-04:002015-05-07T01:30:49.525-04:00We usually use these 1.25mm OD ferrules (they also...We usually use these 1.25mm OD ferrules (they also sell ceramic ones if you prefer): http://www.precisionfiberproducts.com/pfp-lc-1-25mm-od-multimode-304-stainless-ferrules/<br />They sell these in reasonable quantities.<br /><br />best,<br />Jakobjvoigtshttps://www.blogger.com/profile/14632515264267950610noreply@blogger.comtag:blogger.com,1999:blog-2878020709809350448.post-83217587528623395302014-02-06T19:29:59.683-05:002014-02-06T19:29:59.683-05:00Hmm, interesting question. I should clarify that t...Hmm, interesting question. I should clarify that the five major lines do appear segregated between different receptor cells in the bud. For these basic tastes, we have clear connections between human cognitive percepts and the receptor. So I think it's more informative to call them "sweet" cells rather than "sucrose" cells since so many sweet things like sucralose or Mikehttps://www.blogger.com/profile/13460212509225238067noreply@blogger.com